In June, QC’s measuring equipment was extended by a UPLC system. UPLC stands for Ultra Performance Liquid Chromatography and allows for a wide range of applications.
Its main purpose in QC will be the quantification of individual proteins (e.g. Lysozyme and impurities) in a mixture.
The technique is based on column chromatography. It involves a liquid that is pumped with ultra-high pressure through a column filled with adsorbent particles. The liquid transports the sample to be analysed through the column where individual components of the sample are separated from each other due to their different degrees of interaction with the adsorbent particles. A detector then records the signal of each individual component. The higher the detector signal, the higher the concentration of the component.