The AVIDIN-biotin system is a protein-ligand interaction present in nature that has been successfully used in several applications, including the detection of proteins, nucleic acids, and lipids, as well as protein purification. The avidin-biotin system is a simple yet elegant system to link proteins in immunoassays by exploiting the very high affinity of hen egg-white avidin for biotin (vitamin B7).
At Bioseutica, hen egg white AVIDIN, a tetrameric glycoprotein, is purified to homogeneity using an advanced chromatographic manufacturing protocol. This protein binds four biotin molecules per tetramer with dissociation constants approaching 10−15 mol l−1. Its affinity is more significant than most monoclonal antibodies for their haptens.
AFFIAVIDIN is a stable liquid product for several applications. The native protein is chemically modified through successive derivatizations, resulting in a new polymerized and stable molecule with superior affinity to biotin that displays a multiple-fold increase in the detection limit compared with conventional AVIDIN.
AFFIAVIDIN is a modified form of AVIDIN engineered to have a higher binding affinity for biotin by introducing mutations into the AVIDIN protein structure. These mutations increase the number of biotin binding sites or enhance the strength of the interactions between biotin and the protein.
The increased binding affinity of AFFIAVIDIN makes it an even more powerful tool than Avidin for biotechnology applications, as it allows for more efficient and sensitive detection and purification of biotinylated molecules.
AFFIAVIDIN amplifies medium- and low-abundance targets in complex matrices. The high binding affinity and specificity of AFFIAVIDIN make it a versatile tool in various applications that require efficient and specific binding to biotinylated molecules.
Applications
AFFIAVIDIN is commonly used as a tag in protein purification methods. A biotinylated tag is added to the protein of interest and then captured by the high-affinity binding of AFFIAVIDIN to biotin. The protein can then be easily isolated and purified. AFFIAVIDIN can detect and localize specific molecules in cells and tissues. Biotinylated antibodies or nucleic acid probes can label particular molecules. AFFIAVIDIN binds to the biotinylated molecule and creates a signal for detection. AFFIAVIDIN is used in various diagnostic assays to detect the presence of biotinylated targets. AFFIAVIDIN can be utilized in gene delivery by binding to biotinylated DNA or RNA molecules and facilitating cell uptake for gene therapy or genetic engineering applications. In enzyme-linked immunosorbent assays (ELISAs), AFFIAVIDIN coated on a microplate captures biotinylated detection antibodies. A colorimetric or fluorescent signal detects the presence of the target molecule. AFFIAVIDIN can also bind to biotinylated drugs or drug carriers, allowing targeted delivery to cells or tissues with high biotin levels.AFFIAVIDIN has a wide range of applications in various fields, including biotechnology, diagnostics, and research:
Use-cases
- Protein purification
- Immunohistochemistry and in situ hybridization
- Diagnostic assays
- Gene delivery
- Drug delivery
Unit
AFFIAVIDIN available in milliliters ml
Benefits of Bioseutica®’s AFFIAVIDIN
Bioseutica® produces ultra-high purity AFFIAVIDIN, making it particularly attractive for maximizing the efficacy of probes and derivatives, which rely on minimizing background. We provide complete services and technical and application support to help our customers meet their unique product requirements. Our Quality Control Systems ensure reliable performance and quality. Customers are encouraged to request and review our analytical results before shipment for pre-approval as required.
Contact us today to learn more about our first choice, AFFIAVIDIN.
Non-Warranty: The information contained herein is provided in good faith and is true and correct to our knowledge. However, no warranty or guarantee is implied or inferred, and the information may be subject to change without further notice. Bioseutica®’s products are sold with the understanding that the purchaser will conduct tests to determine the suitability of these products for their particular use.
References
- Diamandis EP & Christopoulos TK.: The biotin(strept)avidin system: principles and applications in biotechnology, Clinical Chemistry 1991 | Publisher Site
- Jain A & Cheng K.: The principles and applications of avidin-based nanoparticles in drug delivery and diagnosis, J Controlled Release 2016 | Publisher Site
- Pardridge WM.: Blood-brain barrier drug delivery of IgG fusion proteins with a transferrin receptor monoclonal antibody, Expert Opinion on Drug Delivery 2015 | Publisher Site
- Lesch H et al.: Avidin-biotin technology in targeted therapy. Expert Opin. Drug Deliv. 2010 | Publisher Site
- Stella M et al.: Avidin-Biotin System in Radioimmunoguided Surgery for Colorectal Cancer. Dis. Colon Rectum 1994 | Publisher Site
- Sakahara H & Safa T.: Avidin–biotin system for delivery of diagnostic agents, Advanced Drug Delivery Reviews, 1999. | Publisher Site
- Eakin R. et al.: Egg-White Injury In Chicks And Its Relationship To A Deficiency Of Vitamin H (Biotin), Science 1940 | Publisher Site
- Poissonnier et al.: Observations of the ‘‘Egg White Injury’’ in Ants. PLOS one. 2014 | Publisher Site
- Kunnas et al.: Induction of chicken avidin and related mRNAs after bacterial Infection, Biochimica et Biophysica Acta. 1993 | Publisher Site
- Jain A & Cheng K.: The principles and applications of avidin-based nanoparticles in drug delivery and diagnosis, J Controlled Release 2016 | Publisher Site
- Pignatto et al.: Optimized Avidin Nucleic Acid Nanoassemblies by a Tailored PEGylation Strategy and Their Application as Molecular Amplifiers in Detection, Bioconjugate Chem, 2010 | Publisher Site
